We have developed a semi-automated sequence specific primer (SSP) PCR method for clinical HLA typing and compared the test results with those from a commercial method. METHODS: Primers for each DQA1 and DQB1 allele group were included in our PCR-SSP reaction to allow differentiation of homozygous from heterozygous carriers of risk alleles.

This method, the single specific primer-PCR (SSP-PCR), permits amplification of genes for which only a partial sequence information is available, and allows

We have developed a method that allows the amplification of double-stranded DNA even when the sequence information is available at one end only ( 5 ). This method, the single specific primer-PCR (SSP-PCR), permits amplification of genes for which only a partial sequence information is available, and allows unidirectional genome walking from known into unknown regions of the chromosome. The PCR-SSP-based types of 166 regular blood donors and 148 cadaveric organ donors all showed total concordance with their serologically assigned ABO groups. Six individuals possessing the ABO A subgroups (A3, Ax and Aend) all typed as A1 by PCR-SSP, as expected. PCR-SSP is an appropriate method for ABO typing of cadaveric organ donors and, importantly, enables both ABO and HLA typing to be performed on the same DNA material.

In Methods in Molecular Biology: MHC Protocols (Eds SH Powis, RW Vaughan RW ): This product is optimized for use in the Polymerase Chain Reaction ("PCR") Process which is covered by patents owned by Roche Molecular Systems, Inc. and Improved Method for Factor V Leiden Typing by PCR-SSP. Dear Sir,. Bertina et al . (1) demonstrated that the phenotype of activated protein C resistance was In contrast, in the PCR-SSP methodology (sequence-specific primer – SSP), the discrimination between the different alleles takes place during the PCR process. PCR products from different HLA loci can be analyzed and typed in a few minutes .

This is a method considered fast, efficient and relatively low cost(41).

Application of PCR-SSP method for HLA-B*27 identification as JCI - HLA-B27–mediated activation of TNAP phosphatase Can a positive HLA-B27 test turn

cell fusion (including protoplast fusion) or hybridisation techniques where live cells fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR) it has been established that the presence of Clavibacter michiganensis ssp. Lactobacillus delbrueckii ssp. undan för utvärdering med en kommersiellt tillgänglig HPV-PCR-analys.

For PCR-SSP testing, only those methods which provide DNA of adequate quality and quantity for PCR should be considered, e.g. salting out method (Ref.2). Among the suppliers of commercial DNA extraction kits, suitable products include ”Puregene” from Gentra

In PCR, the size of oligonuleotides used is 18 base pairs, while in assembly PCR lengths of up to 50bp are used to ensure correct hybridization. Red Blood Cell-Ready Gene is inno-train's product line for analysis of erythrocyte blood groups based on the SSP-PCR method. Therefore these diagnostic systems represent the ideal complement to serolgoical blood group typing.

Allelic assignments were concordant for samples 92 and 110, but access to DRB1 typing and the tight allelic linkage within the MHC region supported an alternative haplotype assignment following PCR-SSP. Real-time PCR method for Salmonella spp. targeting the stn gene M.M. Moore and M.D. Feist Seafood Products Research Center, Paciﬁc Regional Laboratory Northwest, US Food and Drug Administration, Bothell, WA, USA Introduction Salmonella spp. are the major cause of food-borne illnes-ses in the United States, accounting for 26% of the hos- Methode Red Blood Cell-Ready Gene ist die inno-train Produktlinie zur Analyse erythrozytärer Blutgruppensysteme mittels SSP-PCR Technik. Damit sind diese diagnostischen Systeme ideal zur weitergehenden Analyse serologisch vortypisierter Proben geeignet.
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Estefanía, E. Facilitation of KIR genotyping by a PCR-SSP method that amplifies Methods used are Molecular biology, FACS, PCR-SSP, PCR-OSSP, and cellular (CDC) method (Treasaki). XenoBiotic Laboratories, Inc., a wholly owned RNA is extracted from respiratory specimens, amplified using RT-PCR and detected using fluorescent generation sequencing or real-time RT-PCR methods [1,11]. Several assays that detect the Staphylococcus aureus ssp.

PCR-SSP technique utilizes oligonucleotide primers to start the PCR that have. de la polimerasa (PCR), tipaje por métodos de ADN, bandas de oligo secuencia especifica (SSO), secuencia de primers específicos (SSP), tipaje basado en HLA typingusing the AllSet+Gold SSP Kits must be performedin the presence of The AllSet+ Gold Kit is a PCR-based method designed to provide low tohigh. to perform HLA typing first by the serological method and to use PCR-SSP as an Recent progress in assigning HLA-class I and class II alleles by techniques This consensus protocol was compiled from the methods used by the participants of the HPA-. 1-5 genotyping exercises organised by NIBSC.
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These have previously been typed with the established method at the clinical Application of a Simple InHouse PCR-SSP Technique for HLA-B* 27 Typing in

Its origins are probably legion, i.e. many people probably thought of it at the same t … We have developed a global intermediate resolution amplification-refractory mutation system (ARMS) PCR-SSP method for distinguishing functionally relevant subgroups of the KIR2DL receptors, as Single Specific Primer-PCR (SSP-PCR): allows the amplification of double-stranded DNA even when the sequence information is available at one end only.

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2 Pulsed coherent radar / PCR radar The invention relates to a method, a device for measuring the posture of a human body and a device

Dear Sir,. Bertina et al . (1) demonstrated that the phenotype of activated protein C resistance was In contrast, in the PCR-SSP methodology (sequence-specific primer – SSP), the discrimination between the different alleles takes place during the PCR process. PCR products from different HLA loci can be analyzed and typed in a few minutes .

Methode Red Blood Cell-Ready Gene ist die inno-train Produktlinie zur Analyse erythrozytärer Blutgruppensysteme mittels SSP-PCR Technik. Damit sind diese diagnostischen Systeme ideal zur weitergehenden Analyse serologisch vortypisierter Proben geeignet.

PCR-SSP is an appropriate method for ABO typing of cadaveric organ donors and, importantly, enables both ABO and HLA typing to be performed on the same DNA material. Single Specific Primer-PCR (SSP-PCR): allows the amplification of double-stranded DNA even when the sequence information is available at one end only. This method permits amplification of genes for which only a partial sequence information is available, and allows unidirectional genome walking from known into unknown regions of the chromosome. This PCR-SSP method is based on the principle that only primers with completely matched sequences to the target sequences result in amplified products under controlled PCR conditions.

These results further suggest that, PCR This polymerase chain reaction/sequence specific primer (PCR-SSP) method gave perfect correlation with serological typing on -1-0 individuals of previously BAGene SSP - reliable molecular blood group typing for few samples or for specific detection tests is the PCR-SSP technique. The kits contain a validated and One hundred and fifty DNA samples were tested by this method, in parallel with PCR-SSP and DNA sequencing for demonstrating the accuracy of the Reverse HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are Commonly used DNA based HLA typing methods include PCR based sequence specific primers (PCR-SSP), and PCR based restriction fragment length Sequence specific primed PCR (PCR-SSP). HLA typing via sequence specific PCR primers is a flexible and still common method, used mostly with clinical specific priming (SSP) (Bunce et al. 1995), polymerase chain reaction-sequence- specific oligonucleotide probes (PCR-SSOP) (Middleton 2000), and sequence-.